538 



FRANK R. LILLIE 



TABLE 3— Continued 



Thus on August 13, I standardized an agglutination solution 

 in sea- water to about 128 agglutinating power and placed 1 cc. 

 in a tube (A). To this was added 1 cc. of a 3.3 per cent sperm 

 suspension; 1 cc. of the agglutinated mixture A was then added 

 to tube (B) containing 1 cc. of the same sperm suspension ; 1 cc. 

 of B was then added to 1 cc. of the same sperm suspension in tube 

 (C). Thus the 128 power agglutinating solution was diluted 

 with 3.3 per cent sperm twice in A, four times in B, and eight 

 times in C. 



(A) 1 cc. 128 agglutinating power solution plus 1 cc. 3.3 per cent sperm 



(B) 1 cc. A plus 1 cc. 3.3 per cent sperm 



(C) 1 cc. B plus 1 cc. 3.3 per cent sperm 



The sperm was then centrifuged off in the thr^ tubes and on 

 test the supernatant fluid was negative in C, fsnntly positive in 

 B and gave a 60 to 80-second reaction in A. On renewed test, 

 B was found almost negative. The result is then that 1 cc. of a 

 128 power agglutinating solution may be fixed bj^ 3 cc. of 3,3 

 per cent sperm. 



Other tests of the binding power of sperm gave results of the 

 same order of magnitude. Thus on August 9, I used a 64 power 

 agglutinating solution as follows: 



