548 



FRANK R. LILLIE 



jelly, and that it is much reduced in nearly all. About 2 per cent of 

 the eggs broken. 



2:33 P.M. washed: Divided in two equal lots in test-tubes J. and B. 

 3:08 P.M.: After settling of eggs poured off sea-water down to 2 cc. in 

 each tube. To A added 6 cc. sea-water. To B added 6 cc. distilled 

 water. Stirred several times until B was thoroughly laked. 



1/80- 



Tests with fresh 1 per cent sperm 



A. 3.40 P.M. 



4 sec. reaction 



B. 4.05 TO 4.15 P.M. 



1/640 — 6 sec. reaction 



1/320 — 7 sec. reaction 



1/160 — 10 sec. reaction 



1/80 — 36 sec. reaction 



1/40 — 8 sec. reaction 1/40 — 60 sec. reaction 



1/20— 13 sec. reaction | 1/20 — 110 sec. reaction 



1/10 — 17 sec. reaction (1)1 1/10 — 22 sec. reaction 



1/1 — 130sec. reaction (1)1 1/1 — 75 sec. reaction 



B. 4.35 p.m. 



1/640— 6 

 1/320- 8 

 1/160—12 



1/80 —25 



1/40 —22 

 1/20 —25 

 1/10 —50 

 1/1 —90 



sec. reaction 

 sec. reaction 

 sec. reaction 

 sec. reaction 

 (chemotaxis) 

 sec. reaction 

 sec. reaction 

 sec. reaction 

 (?) sec. reaction 



The second test of B indicated some progress in the neutrahzation 

 of the fertihzin (cf. 1/20, 1/40, 1/80). The B dilution series is extreme- 

 ly irregular. Reading back from 1/1 (first test) the big jump from 

 1/10 to 1/20 indicates a breaking of the fertihzin X anti-fertihzin 

 combination at this dilution. 



August 24: Test showed some fertihzin present in B. Having poor 

 sperm for indicator, left until next day. Shook up B vigorously; stirred 

 A. 



August 25: B now negative;. 4 strongly agglutinative. 



The distilled water extracts thus show the presence of a sub- 

 stance which combines quickly with the spermophile group of 

 the fertihzin. In the experiment where the jelly was all removed 

 by the prehminary shaking, there was practically immediate 

 neutrahzation of the fertihzin. In the first and third, w^here 

 the fertihzin was present in excess owing to the presence of some 

 jelly, the excess was only slowly combined. Experiments with 

 extracts of dried eggs (August 26) and with destruction of eggs 

 by shaking or grinding also showed the presence of anti-fertilizin. 



