550 



FRANK R. LILLIE 



the quantity of fertilizin decreases, the developmental energy of 

 the fertilized eggs might decrease, owing to relative incomplete- 

 ness of fertilization. This also occurs. In table 3 (p. 536) it 

 will be seen that fertilizations were made at the times of the 11th, 

 16th, 27th and 31st washings. In each case about three drops 

 of both egg-suspensions A and B were taken, including several 

 hundred eggs and fertilized with considerable excess of fresh 

 sperm. It will be recalled that the eggs of lot A had been de- 

 prived of their jelly by shaking, whereas those of lot B retained 

 the jelly. A lost its fertilizin relatively rapidly therefore, at 

 first, but later the repeated washings caused removal of all the 

 jelly in B also. 



Table 4 shows the fertilization results, as regards the percentage 

 of eggs segmenting (200 eggs were counted in each case). There 

 is thus the anticipated decrease in percentage of fertilizations. 

 Moreover, the time between insemination and the appearance 

 of cleavage increases very much, and the eggs cease after a while 

 to produce fertilization membranes and the cells tend to fall 

 apart. 



TABLE 4 



August 21 4:06 p.m. Fert. 1. 



August 22 9:13 a.m. Fert. 2. 



August 22 4:19 P.M. Fert. 3. 



August 23 9:13 a.m. Fert. 4. 



August 23 11:30 a.m. Fert. 5. 



NUMBER OF | 

 WASHINGS 



11 



16 

 27 

 31 

 33 



76.0% 

 97.5% 

 76.0% 

 92.0% 

 83.5% 

 97.5% 

 .39.0% 

 30.0% 

 25.0% 

 24.0% 



segmented 



segmented 



segmented ' 



segmented 



segmented 



segmented 



segmented 



segmented 



segmented 



segmented 



Concomitantly, with these effects of the series of washings, the 

 developmental energy becomes greatly reduced. This was very 

 obvious from the second fertilization. On August 24 (48 hours 

 after fertilization) a large quantity of living material was con- 

 tained in the second A fertilization, but not one had even ap- 

 proximately pluteus structure. The most common form was a 



