560 



FRANK R. LILLIE 



TABLE 7 



is no doubt, therefore, that in eggs with membranes formed by 

 butyric acid the agglutinating substance is fixed, as it is in fer- 

 tiUzed eggs. Presumably, the same would hold true for other 

 parthenogenetic methods which render the eggs incapable of 

 fertilization. 



The next day the washings were continued.. On the sixth, 

 washing A again showed a trace of the agglutinating substance, 

 but came negative again on the remJaining three washings. The 

 reappearance is to be attributed to the few eggs without memt- 

 branes which had been- active all night, accumulating in this 

 time sufficient agglutinating substance to show in the test though 

 not producing sufficient to show in repeated washings. The B 

 eggs came negative in the ninth washing, while the control eggs 

 still gave a 40-second reaction undiluted. 



The B eggs although exposed longer to the butyric acid did not 

 form membranes, and they continued to produce fertihzin longer 

 than the A eggs but in much less quantity than the control eggs. 

 It might therefore, be expected that they would retain capacity 

 for fertilization longer. The fertilization capacities were not 



