574 



FRANK R. LILLIE 



TABLE 12 



It Avill be seen that this sample of blood is very powerful in inhibitor 

 (A series) , and that the agglutinating substance protects from its action 

 at every concentration. The decreases in percentage of segmented eggs 

 in B9 and BIO are to be attributed probably to excess of the agglutinat- 

 ing substance, which was exceedingly concentrated, and which always 

 has some diminishing effect on percentage of fertilizations when the 

 sperm suspension is not too concentrated. In this experiment it will be 

 noted that the agglutinating substance was 6400 strong as contrasted 

 with 1600 on July 21, and the sperm suspension was six times more dilute. 



July 25: (A) Used filtered blood of females; (B) part of A saturated 

 with agglutinating substance by addition of eggs; tested 3200 strong. 

 Fertilizations were made in 0.2 per cent sperm suspensions in the follow- 

 ing dilutions of each, with per cent of segmented eggs as noted, (table 13) : 



TABLE 13 



The same protective action of the agglutinating substance is shown, 

 with some decrease at the higher concentrations due, presumably, to 

 excess of the agglutinating substance. The fertilization B-l actually 

 is better than the co^itrol. 



