105 
XX.—A NEW CASE OF SYMBIOSIS BETWEEN 
A BACILLUS AND A PLANT. 
(Preliminary Note.) 
STUDIES FROM THE PaTHoLoGIcAL Laboratory. 
is 
Dr. Perer Grorcevircu. 
thickness. It is not mobile and forms on agar (in 24 hours, 
at a temperature of 33° (C.) colonies of 1 or 8 mm, in diameter 
which are white in colour and opalescent. Single rods prevail but 
middle of the bacillus there appear two similar chromatic gran- 
ules opposite each other on the lateral walls. By deposition of 
hew chromatic material around these granules towards the centre 
of the bacillus in a diaphragm-like manner the formation of a 
transverse wall is completed. This is deeply coloured by a dilute 
solution of Carbol-Fuchsin and by intra vitam staining (fig 1). 
fter the formation of the transverse wall the bacillus becomes 
attenuated in its middle region, whilst the transverse wall itself 
splits into two laminac. This splitting begins by the division 
This js proved by the fact that two smaller chromatic granules are 
wi . 
verse wall of the new bacilli (figs. 3, 4), whilst the primary trans- 
pe, Wall originates from only two chromatic granules (figs. 1, 
2). The lateral walls of the parent bacillus split at the point 
between the two newly formed laminae of the transverse wall (fig. 
ae ey so the daughter bacilli become separated from each other 
&. 4), 
. The new bacilli formed in this way become more or less oval 
im form. In the protoplasm appears a chromatic granule which 
B 
