106 
fasted or laterall 
i ; and is situated either ai the pole or laterally 
ae litlgs buen tater two chromatic granules are formed * 
aoe t eS 3 
on the lateral walls of the bacillus (fig. 7), and by deposition of 
i i Carbol- 
‘upied (fig. 11). In this stage the rod is not stained by ) 
Fiokaii oF my highly refractive and is yellowish-green in 
olour. 
. SES, . o j 
by the splitting of the lateral wall of the sporangium (fig. 
eich eine becomes sufficiently wide to allow of the escape 
) 
IAC O V¢ OOOOE Cc 
sleleloh carey é) " Ne 
Tn older cultures these free spores prevail and are 23 p in 
length and 1-5-2 » in width. To these adhere the remains of the 
split Sporangia forming a mass which colours deeply with Carbol- 
Fuchsin, while the Spores themselves do not colour with this 
in. 
The wall of the germinating spore takes Carbol-Fuchsin very 
deeply, while its protoplasm is coloured only pale red 
granules in the direction of the pol 
produced (fig. 17). 
“This vesicle, which represents the 
middle of the Spore and in Consequence of its further develop- 
. : plits (fig. 19), the opening becomes 
wider and the vesicle which now i plies Pipi (fig. 20). 
At this stage of development there are in cultures a great 
ower’ Of empty Spore-walls which always show the opening 
through which the embryo has emerged. (fig. 21) 
a 
