Nutrient medium used for all experiments was aqueeys extract of 
lavender shoots. 
126 
RELATION OF SPORES TO TEMPERATURE AND DESICCATION. 
Room temperature approximately 14°—16° 
Per 
t. 
Treatment. Spore. Result. germin 
ation 
ie dr sane of spores in Total number | Number of 7 
sterile water was smeared of spores. germin 
ov ih hg cover slips, 
and 8 air 
They were then placed in| Pycnospore ... 531 0 0 
a oven at 53 Hyaline 
for 17 hours. mo conidium, 327 0 0 
a drop of nutrient medium | Brown ) After 
was d on the centre m. 230 15 
of each cover slip over the | Chlamydospore 123 days. 22 
8 and the cover slips 
inverted over Van Tieghem 
cells, and placed im a dark 
at room tempera- 
Dee a a a A —_$—<—$ 
. No. 0 
spores 
i -of | Total 
Total nes ra ioares Bs 
numbe during | germin- | of spores 
If. we wpores | fixes 17 tre © ene 
suspension of spo pane cea 
water to Langing diops of oven 
nutrient medium on six 
giass cover slips, and th ‘es 2 
inverted over Van Tieghem Pyenospore ... 467 0 0 0 0 
ll Y were placed | Hyaline 
i ffin‘oven at 53° conidium 218 | 0 0 0 0 
for 17 hours, and then re. Bro | atid n 
pred to a dark cupboard conidium. 242 O19 | 27 19 8 
en, eens lamydospore} 153 | 0 |a3}days| 93 | 16 
a 
: Result. 
Total number | Number of spores 
of spores. germinated, 
III, a 
bigamy as in I., but | Pye cnospore .,, 283 57 a4 20 
in an ba sr hours Hyaline ‘7 
0. Tneubator at 39.340 conidium. 320 a |i 
Brown After 70 
conidium. 153 1 27 
Chlamydospore 97 67 days. | 
Beiter on 
No. of 
Total’ | germin- |. No.of | Total 
number ated pe f spores 
of dan germin- | 0 Larch 
spores. | first 17 | 3% ated. 
ours in 
incubator. 
a¥, stray sie in II., but | 
ut | P. yenos r- 4 4 
hanging placed for Hea 193 o. 1) 10 
17 “ in sh "nenbater oh 
at 33-34° C. conidium.| 121 o rr 
es Atte 
onidium, 350 QO {218) 27 
Chlamydospor Te 0 | 34)days. 
BI ne eerie es yoo) Sig anes 
