129 
: Per 
cent, 
Treatment. Spore. Result. germin- 
ation 
No. o ‘otal 
total) er oheae number 
number | ® tea ated i :. é 
nen first — or s 
ger 24 hours} sted 
XIII. Spores subjected to} Pycnospore ...| 265 1 0 1 a4 
the action of artificial | Hyaline : ‘ : 
gastric juice for 12 hours, conidium 123 3 9 
then washed, and placed | Brown 60 a9 
in a hanging drop o conidium 97 64 16 oH re 
vender extract in a Van | Chlamydospore 69 51 3 ‘ 
Tieghem cell at room tem- 
perature. 
XIV. Suspension of spores | Pycnospores ... 521 2 Q 2 44 
in sterile water frozen for 
abo minutes, and 
subsequently covered by 
hanging drop of nutrient 
medium in a Van Tieghem 
cell, and kept at room 
temperature, 
| 
XV. Pycnidia teased from | Pycnospores ... 267.1; Sasha e 103 e 
shoot, and suspended in ; 
water. Then frozen to } 
slide for about 14 minutes / 
icked out with glass hair 
and placed in sterile water 
C ed, a Is j 
transferred to nutrient 
medium ina Van Tieghem | | 
cel | 
Walled brown spore forms is to eliminate their resting ats 
and render them capable of germination in a spleen to aia 
result is apparently identical with that produced w sok re Pent 
= subjected to a temperature below zero. One important pont, 
germinable are still resistant to repeated freezings, but not to 
repeated treatment with pepsin. 
