406 KING. [VoL. XIX. 
irregular nucleolar bodies at one side of the nucleus which 
greatly resemble the structures figured by Carnoy and Lebrun 
as nucleoli resolving into their chromatin constituents. As 
the preparation from which Fig. 44 was drawn was stained 
with iron hematoxylin the nucleolar bodies appear homo- 
geneous and stain very intensely. Their true character is 
shown only when preparations are stained with safranin and 
gentian violet. In such cases these fantastically shaped bodies 
are found to be composed of a number of rounded plasmo- 
somes imbedded in a meshwork of oxychromatin granules 
(Fig. 45). The plasmosomes always appear homogeneous and 
they invariably take the safranin stain; the chromatin stains 
blue and it is always in the form of fine granules which may or 
may not be strung together in a filament. There is nothing 
to indicate that the chromatin in these structures is derived 
from the plasmosomes or vice versa. ‘These peculiar bodies, 
which are found very abundantly in the odcytes of toads with 
a body length of 4-5.5 cm., are unquestionably secondary 
compound-nucleoli which have increased considerably in size 
during the stages of Figs. 39-43 and are now resolving into 
their constituent parts, oxychromatin granules and plasmo- 
somes. Soon after the stage shown in Fig. 44 these irregular 
masses break up, and for a short time the nucleus contains 
a number of plasmosomes surrounded by chromatin granules 
(Fig. 46). Ata later period the oxychromatin granules sep- 
arate from the plasmosomes and scatter thoughout the karyo- 
plasm, and for the first time since before the synizesis stage 
the nucleus has all of its chromatin separated from the plas- 
mosome substance. 
As I was unable to obtain any young toads in the fall with 
a body length of over 5.5 cm., I have not been able to follow 
the later changes in the odcytes in the ovaries of young fe- 
males, and I have had to make use of the odcytes developing 
in the ovaries of adults to complete my study of the odgenesis 
of Bufo. If adult females are killed soon after the breeding 
season in April or in May the ovaries are found to be filled 
with young ova, many of which contain nucleolar bodies simi- 
