7 
ie KING. [Vou. XIX. 
that the greater number of the nucleoli lie in the part of the 
nucleus that is nearest the periphery of the egg. It hardly 
seems probable that this arrangement would be found so con- 
stantly if it had no significance. It seems to me a possibility, 
at least, that the accumulation of most of the nucleoli in one 
part of the nucleus may have something to do with the move- 
ment of the nucleus towards the periphery of the egg. This 
arrangement of the nucleoli strongly suggests also that the 
polarity of the egg is determined at or soon after synizesis, 
since the location of the largest of the compound-nucleoli, 
from which the greater number of secondary compound-nucle- 
oli are derived, indicates the part of the nucleus which will be 
nearest the animal pole in the later growth stages of the 
oocytes. 
After the nucleus has reached its final position at the per- 
iphery of the egg there is a rearrangement of the nuclear con- 
tents so that the nucleoli become distributed fairly evenly 
around the nuclear periphery, while the chromosomes and the 
remains of the oxychromatin filaments are found in the centre 
of the nucleus (Fig. 49). Whether this arrangement is due 
to the activity of the nucleoli themselves, I have not been able 
to determine. These bodies always appear round and they 
never show processes similar to those found by Leydig and 
also by Eimer (26) and considered by these investigators to 
_be the means through which the nucleoli change their position 
in the nucleus. It is at this stage of development shown in 
Fig. 49 that the nuclear membrane is most irregular in outline, 
and it is very probable that this irregularity is due to the 
close proximity of the nucleoli. As a rule all of the oxy- 
chromatin filaments have disintegrated at this time; the chro- 
mosomes can always be found in favorable preparations, al- 
though they stain very faintly. 
Several investigators, among whom may be mentioned Will 
(92), Fick, and Leydig, maintain that at or before the stage 
of Fig. 49 nucleoli pass out of the nucleus into the cytoplasm 
where they either dissolve or take part in the formation of the 
yolk. Although I have examined a large number of eggs in 
