20 KING. [VoL, XIX, 
where they “im losgelésten Zustande die Form und Natur 
kleiner AmOben zeigen.” Subsequently they pass through the 
nuclear membrane into the cytoplasm and move to the per- 
iphery of the egg where they form groups of granules which 
develop into yolk. 
Bataillon (6) also derives the yolk in the amphibian egg 
from the substance of the nucleus, but he believes that it is 
formed from the chromatin. ‘Des massules chromatiques 
issues de la vésicule germinative viennent donner dans le 
plasma ovulaire et a la périphérie d’abord, de véritables élé- 
ments cellulaires transitoires dont ils fournissent le noyau, et 
prendre part a la formation simultanié des tablettes vitellines 
et du pigment.” 
As a result of a study of the ovarian egg of Rana and of 
Necturus, Macallum (64) concludes that the peripheral 
chromatin nucleoli generate a substance which diffuses grad- 
ually through the nucleus into the cytoplasm. “I regard the 
yolk spherules as formed by the union of a derivative of the 
nuclear chromatin with a constituent of the cell protoplasm.” 
Support for this view is furnished by the more recent cyto- 
logical studies of Carnoy and Lebrun (15). These investi- 
gators state that the greater number of chromatin granules 
that are produced by the resolution of nucleoli are not used 
in the formation of a new generation of nucleoli, but that they 
are dissolved in the achromatin substance of the nucleus and 
transformed into nucleinic acid. This acid passes by osmosis 
through the nuclear membrane and is diffused through the 
cytoplasm. “Dans les plages formatrices, il rencontre les 
globulines de réserve imbibées d’eau, et se combine avec elles 
pour former la paranucléine d’abord, la vitelline en suite... . 
Nous considérons les plaques vitellines comme étant des pro- . 
duits de l’activité du noyau et du cytoplasme: celui-ci fournirait 
les globulines, le noyau, l’acide paranucléinique. Les vitel- 
lines sont, en effet, des paranucléo-albumines, c’est-a-dire des 
combinaisons de paranucléine avec un albumine qui est ici 
une globuline.”’ 
Jordan has observed in the newt appearances which might 
be interpreted as a migration of very minute granules from 
