Hatar, Spenal Ganglion Cells in the Rat. 3 
Fluids devised by the author. 
6. Corrosive sublimate, Sat. sol. in formalin 30 cc 
Glacial acetic acid : : : ee SOICe 
Normal salt solution ; : : 5) OR Nte 
7+ Picric acid, sat. sol. in 10% formalin - 60cc 
Glacial acetic acid . : : 5 2 OFCe 
Corrosive sublimate : : : Zoe 
In the above tables, the fluids which were recommended 
by the previous authors were used to preserve the materials 
according to the directions given. The other fluids which were 
new were employed in the following manner: 
The tissue should remain in these fluids for 6 to 12 hours, 
according to the size of the piece. Generally thin pieces give 
more satisfactory results. After fixing, the tissue should be 
transferred to running water where it should remain 4 to 5 
hours. Then it should be transferred to weak alcohol, about 
30%. The further procedure is the same as for ordinary 
paraffin embedding. 
As staining agents, all of the following were used on each 
piece of tissue: 
1. Toluidin blue, aq. sat. sol. 
2. Thionin, aq. sat. sol. 
3. Nussv’s fluid—as follows; 
Methylene blue (powder) (methylene B. pat.) 1.3 grms. 
White castile soap (Venice soap). ; 0.7 grms. 
Distilled water Pane: ; ; wo 332.05 Ce; 
For counter-staining : 
1. Erythrosin 1% in 95 % alcohol. 
2. Eosin 1% in 80% alcohol. 
As the clearing agents : 
Ie 42x ylol. 
2. Cedar oil. 
ray comparison of sections prepared by the methods named 
above gave the following results. In each case, NIssv’s stain- 
able substance and the non-stainable substance came out very 
beautifully. The materials fixed with Gitson’s fluid show greater 
shrinkage than those prepared by the other fluids. Grar’s fluid 
