288 JOURNAL OF COMPARATIVE NEUROLOGY. 
chromatic figure is exactly similar in its principal characters to 
that of the preceding stage (Figs. 16, 17). In this stage, the 
nuclear membrane has not yet formed and the nucleolar sub- 
stance surrounds the figure thickly in an irregular manner. In 
a far more advanced stage, however, the chromatic figure mod- 
ifies itself in a remarkable manner, presenting a spiral arrange- 
ment along the periphery of the nuclear membrane (Fig. 20). 
The continuous chromosomes which present a spiral arrange- 
ment are constricted at intervals along their course, and divide 
the spiral line into numerous small segments. The linin could 
not be distinguished from the nucleolar substance. This stage 
is shown by Fig. 20. Following changes in the chromatic sub- 
stance, the nuclear substance re-integrates and| produces num- 
erous acidophile granules (Fig. 21). In the next following stage 
the spiral arrangement of the filament ceases to be visible, but 
instead of that a reticular arrangement reappears in the nuclear 
area. The chromatic granules which were constricted from the 
continuous spiral line, diminish in size to a considerable degree 
and remain suspended in the reticulum (Figs. 21-23). 
The filaments which form the reticulum just mentioned are 
not the same substance that forms spiral thread (Fig. 20) ; that 
is, the nucleolar substance which forms part of the sheath of 
the chromosome in the preceding stage—not shown in Fig. 20 
—forms scattered granules which, like the chromatic granules 
are suspended in the reticulum. The reticulum itself is com- 
posed entirely of linin substance. The scattered acidophile 
particles (nucleoli) are always larger than that of chromatic 
granules and further they stain a deep blue in the preparations 
made by HERMANN’s method. This stage is shown in Fig. 21. 
Large spherical bodies are distinguishable here and there in the 
figure which represent the scattered acidophile particles of nu- 
cleolar substance. These scattered acidophile particles, how- 
ever, sooner or later are collected (Fig. 22) at one point in the 
nucleus, generally at the center, although, in many cases, they 
remain in the scattered form, and do not centralize. Or, very 
frequently, some of these scattered acidophile particles central- 
ize themselves while the rest of them remain in their original 
Ce a 
