Hata, MMitoszs in Nerve Cells. 291 
forming a complete circle of the nucleolar substance (Fig. 22, 
23). In the adult stage, more processes are produced. These 
in turn fuse until there is formed a very complicated network. 
This latter phase of the nucleolus can easily be seen in the 
nucleolus of the resting nucleus (Figs. 25, 26, 27). 
Sooner or later, after the acidophile particles have been 
accumulated at one point within the nucleus, the basophile sub- 
stance as well as the linin surrounds the acidophile particles 
intimately. These three different substances, which are cen- 
tralized in the nucleolus, can be beautifully demonstrated in the 
embryonic tissues which stain properly. This preparation shows 
us the acidophile substance staining red; the linin a faint red 
and the chromosomes a deep blue after HERMANN’s method. 
The nucleolus of the adult nucleus does not show these dis- 
tinctions clearly, since the basophile substance accumulates 
about the periphery of the acidophile particles in great quan- 
tity and obscures them. 
Fig. 25 isa nucleolus taken froma spinal ganglion cell 
of the gray rat. The material was preserved with the au- 
thor’s sublimate mixture,’ followcd by toluidin blue and ery- 
throsin. In this case the chromatic granules, as well as nucle- 
olus asa whole, stain bluish, while the acidophile particles 
stain deep blue. The number of the granules is variable even 
in the same kind of cells. 
Fig. 26 is a nucleolus of the same animal preserved with 
Carnoy’s fluid followed by the same counter-stains. In this, 
the granules are much more numerous than the former. Fig. 
27 isa nucleolus taken from the efferent neurone of Torpedo 
occidentalis which was preserved with 10% formalin stained 
with toluidin blue and erythrosin. In this case, we can see the 
similar granules in the nucleolus. Von LENHOssEK!' distin- 
guished a group of from three to five punctiform strongly 
z HATA, S.: The Finer Structure of the Spinal Ganglion Cells in the White 
Rat. Journ. Comp. Neurol., Vol. XI, No. 1, 1901. 
1 y. LENHOssBK: Die feinere Bau des Nervensystems im Lichte neuester 
Forschungen, Berlin, 1895. 
