292 JOURNAL OF COMPARATIVE NEUROLOGY. 
stainable particles in the nucleolus which he termed ‘‘endo- 
nucleoli,”’ but he makes no mention of their significance. Our 
figures of the internal structure of the nucleolus correspond 
exactly to that of von LENHOsSEK and therefore we identify the 
acidophile particles with the endonuclei of von LENHOSSEK.? 
The following is a brief sketch of the investigations on the 
structure and staining reactions of the nucleolus. 
FLEMMING? regards the nucleolus as composed mostly of 
the chromatic substance. 
Rosin,* who employed Bronnp1's stain, maintained that the 
nucleus as well as nucleolus are neutrophile. 
Ramon y Cajat * maintains that the nucleolus is composed 
entirely of nuclein which, however, was modified in the central 
portion by long mitotic repose. Lrvi° has shown that the 
central part of the nucleolus stains with the acid color, while 
the periphery of the organ takesa basic color. From the above 
fact he concluded that the nucleolus of the nerve cells is com- 
posed of two entirely different substances; one of these is aci- 
dophile and the rest is basophile. 
VAN GEHUCHTEN’ holds the same view as CajAL, maintain- 
ing that the nucleclus is composed entirely of chromatin. 
BUu ier’ described the nucleus of the spinal ganglion cells of 
1 LEVI was unable to see these granules, which were referred to by v. 
LENHOSSHK, in his preparation (See Alcune particularita di struttura del nucleo 
delle cellule nervose: Rev. di. patol. nerv. e. ment., 1896, f. 4.) 
2? FLEMMING: Bau der Spinalganglienzellen. Festgabe f. Henle, Bonn, 1882. 
8 Rosin: Ueber eine neue Farbungsmethode des gesammten Nervensystems 
nebst Bemerkungen iiber Ganglienzellen und Gliazellen. Neurol. Centralbl. 
Leipaig, Bd. xii, 1893. ; 
* RAMON y CaJAL: Estructura del protoplasma nervioso. ev. tremest. 
Microg., Madrid, Vol. 1, 1896. 
5 Levi, G.: Considerazioni sulla structura delle nucleo delle cellule nervose. 
Rev. pat. nerv. e ment., III, 289-296, 1§97. 
6 VAN GEHUCHTEN: L’anatomine fine dela cellule nerveuse, MVeurolog. 
Centralbl., 1597, p. 905, and Revue Neurologique, 1897, p. 494. 
‘BUHLER: Untersuchungen itiber deu Bau der Nesvenzellen. Verh. der 
Physth, med. Gesselsch. zu Wiiraburg. Bd. XXXI1, Nr. 8, ’98. 
