REACTIONS OF AMEBA TO PROTEINS 59 



with the globuhn. But when both prongs came into contact 

 with the globuhn (944) they started to surround it. The ameba 

 was at this time in the form of a ring (945). The tips of the 

 prongs fused as the globuhn was ingested. The globulin was 

 ingested in a food cup formed chiefly by the left prong. After 

 ingestion the ameba rested for about twenty minutes, then 

 moved off. Several similar instances have been observed. 



Reactions to large pieces of globulin 



In the experiments recorded above fragments of globulin small 

 enough to be easily eaten were always employed. Let us now 

 consider some experiments in which the globulin grains in every 

 case were too large to be eaten. 



In the first trial (408) the globulin grain w^as definitely avoided. 

 The globulin w^as then shifted (412). As the ameba moved 

 directly toward it, the antero-posterior diameter foreshortened, 

 while the anterior edge broadened out considerably. Before 

 the ameba came quite into contact with the globulin, a huge 

 food cup large enough to enclose the test object was started 

 (416). The ameba moved forw^ard into contact with, and laid 

 the partly formed food cup over the grain of globulin, to which 

 the ameba then adhered with but shght movement for several 

 minutes. After this period of comparative quiet, the ameba 

 became more active in its movements and less of its body was 

 in contact with the globulin. Pseudopods were then sent out 

 successively in various directions (422 to 426) only to be re- 

 tracted later. Finally one was sent out (429) which led the 

 ameba away from the globulin, but only after the globuhn had 

 been dragged for a considerable distance. The ameba was in 

 contact with the globulin for an hour and nine minutes. One 

 minute later the globulin was shifted (433). The ameba flowed 

 directly into contact w^ith it, and remained so for eleven min- 

 utes. There was no attempt at the formation of a food cup, 

 nor was the globuhn adhered to with avidity. On the fourth 

 trial the ameba avoided the globulin (445). On the fifth trial 

 (450) the ameba moved into contact Avith the globulin and re- 



