60 A. A. SCHAEFFER 



mained in very loose contact, without at any time quieting 

 down, for fourteen minutes. On the sixth trial the ameba 

 moved into contact with the globuhn at its side (457). The 

 ameba remained in rather loose contact with the globulin for 

 about twelve minutes. The same piece of globulin was, for the 

 seventh time, placed ahead of the ameba (467). As the ameba 

 moved forward it broke up into several pseudopods, indicating 

 negative behavior; but presently one of them moved forward 

 some distance, then turned to the left and toward the globulin, 

 and finally moved into contact with it. The ameba remained 

 in loose contact with the globulin for about six minutes. 



A half minute later a new piece of globulin was laid in the 

 ameba's path (482). At first the ameba reacted decidedly 

 negatively and mo^-ed on past the globuUn. But a pseudopod 

 was then sent out on the right which was directed toward 

 the globulin and which carried the ameba into contact with 

 it. The globulin attracted the ameba strongly but not so 

 strongly as did the other piece in the first trial. The ameba 

 remained in contact with the globulin for about thirty-seven 

 minutes. For the sake of comparison a small piece of fresh 

 globulin was laid near the ameba, now in Y-form (496). The 

 globulin was ingested promptly without the formation of a food 

 cup. 



Twenty-three hours later the ameba was found to be in normal 

 condition. A large piece of fresh globulin was laid in its path 

 (502). The ameba reacted negatively at first (503) but it hap- 

 pened that as the ameba toppled over (it had been standing on 

 end) it came near the globuhn to which it was then strongly 

 attracted. The ameba had been in contact with the globulin 

 for an hour and twenty-two minutes when observations were 

 terminated. A number of times the ameba had almost broken 

 away, but each time it returned again to the globulin. 



These observations on the behavior toward large food masses 

 show clearly: (1) that the size of the food object plays little part 

 in selection; (2) that globuhn has the power of initiating the 

 formation of a food cup before the ameba has come into contact 

 with it; (3) that the globulin particle becomes changed by being 



