572 MARY J. HOGUE 



SUMMARY 



1. An amoeba limax was grown on agar media of different 

 densities, made up with Witte's peptone. . 



2. On 2.5 per cent agar the amoebae are rather circular, with 

 few pseudopodia. Compared with those grown on the thin 

 medium they are more numerous and often massed together. 



3. On 0.5 per cent agar the amoebae are elongated, with 

 irregular pseudopodia. They move freely on this medium and 

 are evenly distributed over it. 



4. On 1, 1.5, and 2 per cent agar the amoebae show the forms 

 intermediate between the amoebae grown on 0.5 and 2.5 per* 

 cent agar respectively. 



5. The media best adapted to the growth and multiplication 

 of the amoebae are 1.5 and 2 per cent agar plus 0.4 per cent 

 Witte's peptone. 



Wellesley, Mass., 

 October 4, 1916. 



LITERATURE CITED 



Burrows, M. T. 1913 The tissue culture as a physiological method. Trans. 



of the Cong, of American Physicians and Surgeons, 9. 

 HoGUE, M. J. 1914 Studies in the life history of an amoeba of the Limax group. 



Arch. f. Protistenk., Bd. 35. 

 Jennings, H. S. 1904 Contributions to the study of the behavior of the lower 



organisms. Carnegie Inst, of Washington, Pub. No. 16. 

 ScHEEL, C. 1899 Beitrage zur Fortpflanzung der Amoeben. Festschrift zum 



Siebenzigsten Geburtstag von C. V. Kupffer. 

 Uhlenhuth, E. 1915 The form of the epithelial cells in cultures of frog skin, 



and its relation to the consistency of the medium. Journ. exp. med., 



July, vol. 22, no. 1. 

 Wherry. W. B. 1913 Studies on the biology of an amoeba of the Limax group. 



Arch. f. Protistenk., Bd. 30. 



