324 



EDWARD C. DAY 



Siphons of three specimens 



PROTOCOL 5 



of Ciona were amputated as indicated in figure 4 by 

 dotted lines 



April 17, 1913 



April 17, 1913 



April 17, 1913 



April IS, 1913 



April 19, 1913 



12.20 P.M. 



12.30 P.M. 



4.45 P.M. 



12.15 P.M. 



4.45 P.M. 



CIONA NUMBER 7 



Siphons ampu- 

 tated 



Piece A, no re- 

 action 



Piece B, irregu- 

 lar automatic 

 contractions 



A, reacts to tac- 

 tile stimulus 



CIONA NUMBER 8 



Siphons ampu- 

 tated 



Piece A, no re- 

 action 



Piece B, no re- 

 action 



Piece A, reacts 

 to tactile 



stimulus; also 

 automatic 

 contractions 



N.B.— 7A and 8A have long 

 latent period. 8A reacts locally 

 to stimulation of lip-lobe; often 

 required repeated stimulations 

 to elicit a response. 



A, no reaction 



B, feeble re- 

 sponse to tac- 

 tile stimulus. 



A, reacts feebly 

 to strong 

 stimulus. 



B, reacts to 

 strong stimu- 

 lus. Cut sur- 

 faces of body 

 also reacts 



A, automatic 

 contractions 



B, reacts to tac- 

 tile stimulus 



A, reacts to tac- 

 tile stimulus; 

 also auto- 

 matic con 

 tractions 



B, reacts to 

 stimulus 



CIONA NUMBER 9 



Siphons ampu- 

 tated 



Piece A, no reac- 

 tion 



Piece A, no reac- 

 tion 



A, reacts to tac- 

 tile stimulus ; 

 also automatic 

 contractions 



B, reacts to tac- 

 tile stimulus 



A, automatic con- 

 tractions 



B, reacts to tac- 

 tile stimulus 



A, reacts to tac- 

 tile stimulus 



B, also reacts 



