EVIDENCE OF GERM CELL SELECTION 391 



In this way it was possible to recover 42 cc, lea^dng a balance 

 of 24 cc, as the approximate amount that had evaporated. 

 The absolute alcohol vaporized into the tank therefore prob- 

 ably ranged between 20 cc, and 25 cc. for treatments last- 

 ing about an hour. There was alwaj^s a rise in temperature 

 amounting to about 3*.5°C. dming an hour treatment (e.g., on 

 May 21, from 26'' to 29.5^), and this of course facilitated evapora- 

 tion of the alcohol. The treatments were usually begun about 

 9 A.M. and 5 p.m. Then- dm^ation varied considerabl}^, as wdll 

 be noted below. 



With the hens used in experiment 4 a slightly different con- 

 tainer was employed for administering the alcohol. Enameled 

 specimen jars about 15 inches square and approximately 12 inches 

 high, with close-fitting covers, were found to have a capacity 

 of 1.6 cubic feet. These jars were used as inhalation chambers, 

 and one or two hens placed in each after an excess of alcohol 

 had been allowed to evaporate in them for from twenty to 

 thirty minutes. 



REACTIONS OF THE ALCOHOLIZED FOWLS 



The degree of alcoholization obtained is perhaps best indi- 

 cated by the observed responses of the birds to this treatment. 

 While a certain amount of resistance or accommodation was 

 acquired as the treatment progressed, it was apparent that the 

 males were throughout less affected than were the females. The 

 alcohol absorbed on each occasion was sufficient to cause at 

 least a mild degree of intoxication. Since the reactions were 

 very constant, the details of a single treatment may be given 

 as illustrative of the typical behavior of one of the males fol- 

 lowing the inhalation of alcohol vapor. 



On May 29, 1917, the inhalation chamber was prepared as usual 

 and male no. 8 treated from 10 a.m. to 11.15 a.m. During the whole 

 time he sat on the false bottom turning his head only when disturbed 

 by objects seen through the window. When undisturbed the front of 

 the head rested against the side of the jar. The nictitating membranes 

 passed over the eyes at the rate of thirty times per minute. Notes 

 on respiration were made as follows : 



