392 C. H. DANFOKTH 



Time Rate Remarks 



10.15 40 per minute Shallow. 



10.25 25 per minute Deeper. 



10.50 22 per minute Deep, regular. 



11.15 22 per minute Deep, but irregular. 



At the end of the treatment the bird was breathing noisily and with 

 difficulty. The mouth was open. 



11.15. Removed from the tank. Quite passive. Did not struggle. 



11.17. Returned to the breeding pen. Stepped very high, but 

 not unsteadily. Showed indications of hyperexcitabihty. Shied at 

 a feather and jumped out of the way of a chick that approached 

 from behind. The labored breathing improved rapidly. 



11.20. Picked up pieces of grass and called hens. Very gallant. 



11.22. Crowed repeatedly but abnormally. 



11.25. Ate heartily when the flock was fed. 



This behavior was quite characteristic of the males follow- 

 ing both morning and afternoon treatments. The ability to 

 crow was always affected. Occasionally one was unsteady 

 on his feet and they frequently misjudged distances in attempt- 

 ing to jump or fly. Repeated rubbing of the eyes on the back 

 was characteristic, but despite the severity of the treatment, 

 no whitening of the conjunctiva was noted. 



As a control for the above-described behavior, on the after- 

 noon of the same day the inhalation chamber was again thor- 

 oughly cleaned and aired and the same male treated exactly as 

 before except that no alcohol was used. He was kept in the 

 chamber from 3.10 to 3.45 p.m. During the first half of this 

 period he sat quietly on the bottom of the container, but during 

 the latter half he stood, possibly owing to the gradual exhaustion 

 of oxygen. Respiration fell from 42 to 30 per minute. The 

 moment the cover was removed from the tank he made violent 

 attempts to escape, struggling and squawking. (Nothing of this 

 sort ever happened following an actual treatment.) Returned 

 to the breeding pen he displayed none of the customary excit- 

 ability, high-stepping and gallantry towards the hens. After 

 a regular alcohol treatment given an hour later he again showed 

 the usual reactions indicative of intoxication. 



It will be seen from the foregoing that the immediate phys- 

 iological effect of the alcohol upon the males, while unmis- 



