OXYGEN AND MALE PRODUCTION 



471 



and placed in a pan of running water in the direct sunlight. 

 The running water maintained a temperature of 20° to 25°C. 

 The old stable-tea culture water had been standing in a north 

 light for several months and had only a small amount of free 

 oxygen in it. This made an excellent starting solution. Usually 

 after a short time in the sunlight the Chlamydomonas would 

 emit free oxygen in sufficient quantities to rise to the surface in 

 minute bubbles. After several hours in the sunlight there was 

 formed usually a frothy scum on the surface which was composed 

 of these minute bubbles of oxygen. 



Table 1 shows some of the details and the results of these few 

 experiments. Experiments 1 and 3 show the amount of oxygen 

 generated in periods of one hour, two hours, and four hours, 



TABLE 1 



Shoiving that in sunlight the green flagellates, Chlamydomonas, give off considerable 



quantities of free oxygen in old stable culture water that is devoid of all 



food substances 



