72 M. F. GUYER AND E. A. SMITH 



normal saline solution. On each date each fowl received 1.5 cc. of 

 this mixture, containing approximately one-half of a lens. 



On December 6 one of the fowls was killed and bled. On December 

 7 the blood which in the meantime had been kept in the dark at a tem- 

 perature of about 5°C. was centrifuged and the decanted serum used 

 for injection into the two pregnant rabbits, each of which, between 10 

 and 11 a.m., was given 6 cc. of the. undiluted serum (table 2). The in- 

 jection was made directly into the blood stream through the marginal 

 vein of the ear. By 2.30 p.m. each rabbit showed evidence of illness, 

 particularly individual A, which was passing what appeared to be 

 bloody urine at frequent intervals. Both had apparently recovered 

 by the next day. 



December 9. Both rabbits were given a second injection of serum 

 from another of the sensitized fowls which had been killed on De- 

 cember 8. This time the dose for each rabbit was changed to 5 cc. of 

 serum diluted with 3 cc. of normal salme solution. The animals 

 showed no ill effects after this treatment. 



December 11. Treatment similar to that of December 9 was given, 

 the serum being from the same source. 



December 14. Each rabbit was injected with 6 cc. of the undiluted 

 serum of a third one of the sensitized fowls, and this dose was repeated 

 on December 15, using serum from the same fowl. 



December 18. Each rabbit was given 6 cc. of undiluted serum from 

 the fourth fowl. 



December 30. Rabbit B, an Albino, gave birth to seven young. 

 When the young finally opened their eyes, one had a shght opacity of 

 about one-half of the lens of the left eye. This cleared up after three 

 days. A second one had the entire left eye noticeably smaller, with the 

 lens opaque. This opacity has persisted as has also the smaller size 

 of the eye. At the present writing (October 15) this individual is 

 about fully grown. While its right eye seems to be perfectly normal, 

 the left is but little larger than that of a newly born rabbit. A third 

 individual had a cloudy rim around the edge of the lens which slowly 

 cleared after several days. 



The four other young, in so far as one could judge from external 

 appearance, had normal lenses. But since young of another mother 

 subjected to similar treatment had watery or liquefied lenses (see ex- 

 periment 5), a condition which was not detectable until the young were 

 killed and the eyes dissected, it is possible that cytolytic effects existed 

 in the lenses of some of these four apparently normal individuals. 

 One, in fact, when killed four months later (experiment 5) was found 

 to have a watery and diffuse lens. By that time, unfortunately, two 

 of the other three had been disposed of so that it was impossible to 

 determine the consistency of their lenses. The last one is being kept 

 for further breeding experiments. She is at present mated with the 

 dwarfed-eyed one. 



Rabbit A failed to bear young. Since she is the one recorded as 

 passing what appeared to be bloody urine after the first injection, it is 



