74 M. F. GUYER AND E. A. SMITH 



not impossible that she aborted at this time. She was remated suc- 

 cessfully later and is the subject of experiment 2. 



Experiment 2 



Rabbit A of the former experiment was mated on January 10. She 

 was not further injected with sensitized serum, as it was desired to find 

 if the effects of the earlier injections might hold over and affect the 

 young in utero. On February 12 she gave birth to eight young. None 

 showed any perceptible effect of the lens cytolysin administered to the 

 mother during the earlier experiment. 



Experiment 3 



In this experiment seven fowls were used for sensitization, and two 

 pregnant rabbits, C and D. January 17, six lenses of adult rabbits 

 were ground up in a mortar and diluted with 20 cc. of normal saline 

 solution. Each of the seven fowls was given a 2.5 cc. dose of this 

 suspension intraperitoneally. This treatment was repeated January 

 19 and again January 22.* Two of the fowls were bled January 30 in 

 the usual way, and on January 31 the two rabbits, C and D, were each 

 injected through the ear vein with 10 cc. of a mixture of serum 7 parts 

 and normal saline solution 3 parts. On February 3 serum was taken 

 from another of the sensitized fowls, and the two rabbits were each 

 given a 10 cc. injection of a mixture of the serum 8 parts with 3 parts 

 of normal saline solution. This was repeated on February 7 with se- 

 rum from yet another treated fowl, using the same proportion of serum 

 and normal saline solution as on February 3. On February 15 rabbit 

 C bore five young. All were apparently normal at birth, though one 

 rapidly developed abnormally large incisor teeth and had to be killed 

 April 24 to prevent its slow starvation. The eyes of all were appar- 

 ently normal. Rabbit D bore four young February 16, all with nor- 

 mal eye structures as far as could be judged from external appear- 

 ances. The young of both of these experiments had been disposed of 

 before the importance of determining the consistency of their lenses 

 was realized. 



Experiment 4 



Rabbit B of experiment 1 was mated again January 24 to January 

 30. She was injected at intervals with serum from the sensitized fowls 

 used in experiment 3, namely, on February 3 with a mixture of 7 cc. 

 of serum and 3 of normal saline solution ; on February 7 with a mixture 

 of 8 cc. serum and 2 cc. of normal saline solution, and on February 13 

 with a similar dose. On March 6 she gave birth to six young which 

 ranged in size from an extremely small one to one considerably larger 

 than the others. No eye defects were visible. Again, because the eyes 

 were of normal size and transparency, the young were disposed of, 

 unfortunately, without determining the texture of the lenses. 



