STUDIES ON CYTOLYSINS 75 



Experiment 5 



Five fowls were injected March 8, 15, 17, and 20, respectively, with 

 lenses from young (about half-grown) rabbits, four lenses being used 

 each time, so diluted with normal saline solution that each fowl re- 

 ceived 2.5 cc. of the mixture. Rabbit E was injected in the usual 

 way with serum from these fowls, the doses being: 



March 31, 10 cc. of a mixture of 10 parts of serum and 2 parts of 

 normal saline solution. 



April 3, 10 cc. of a mixture of 10 parts of serum and 2 parts of 

 normal saline solution. 



April 5, 11 cc. of a mixture of 10 parts of serum with 2 parts of 

 normal saline solution. 

 On April 24 a litter of eight young was born. All were tardy in 

 getting their ej^es open, one being particularly delayed in this respect. 

 The eyes of all when opened seemed weak and watery. By May 17 

 all eyes seemed normal and most of the young were given away as 

 pets in order to reduce the number of individuals which must be car- 

 ried over the summer by a caretaker. On May 19 one remaining 

 young one of this litter was killed. An examination of its eyes re- 

 vealed the fact that its lenses were liquid, though still transparent. 

 The lenses of three normal rabbits of the same age were examined and 

 found to be fibrous and fairly firm. This suggested that the young in 

 the litters of the other females which had been treated with serum 

 might also have had lenses similarly affected though not visible from 

 the exterior. Unfortunately, as already recorded, these had been 

 disposed of before the discovery was made. One male of the litter 

 produced in experiment 1 was still available. It was killed May 19, 

 about four months after birth, and its lenses were found to be very 

 watery and diffuse when compared with those of normal rabbits of the 

 same age. 



Since this same liquefaction of lens was found in the lenses of 

 young mice in similar experiments which were being carried on 

 by the senior author in California in the meantime (cf. section 

 III), there seems no reason for doubting that it is an effect pro- 

 duced by a foreign serum sensitized to crystalline lens. A new 

 series of experiments is being undertaken for further enlight- 

 enment on this point. 



None of the adult females used in the foregomg experiments 

 showed in the lenses of their own eyes any effects of the treat- 

 ment. The tabulated data of the experiment are shown in 

 tables 1 and 2. 



