FEEDING SUDAN III TO YOUNG ALBINO RATS 



111 



TABLE 5 



Showing the percentage of ivater in the various organs of Sudan HI fed rats con- 

 trasted tvith those of control rats 



Body length 

 Body weight 

 Age, days. . . 



Blood. 



Brain 



Heart 



Kidneys .... 



Liver 



Spleen 



Lungs 



Pancreas .... 

 Thymus 



Control 



lcf+19 



129.0 

 52.0 

 "48 

 81.6 

 79.5 



76.8 

 73.1 

 77.5 



72.4 



Sudan 

 III 



20^+19 



102.7 

 26.7 

 48 



83.0 

 79.0 



76.0 

 67.1 



77.5 



76.9 



SERIB8 III, V 

 AND VI 



Control 



lcf+29 



153.0 

 99.0 

 63 



80.7 

 79.0 

 77.5 

 75.8 

 70.9 

 77.2 

 78.2 

 67.9 

 78.6 



Sudan 

 III 



2cf+19 



120.2 

 46.4 

 63 



83.0 

 78.6 

 77.6 

 75 4 

 68.2 

 76.7 

 80.2 

 75.1 

 80.9 



SERIES VII 

 AND VIII 



Control 



29 



146.5 

 79.8 

 58 

 80.9 

 79.0 

 77.0 

 76.3 

 71.3 

 78.1 

 79.2 

 69.9 

 79.7 



Sudan 

 III 



29 



110.0 

 36.8 

 58 



80.8 

 78.6 

 76.8 

 75.3 

 67.2 

 76.5 

 80.0 

 74.6 

 80.5 



SERIES IX 

 AND X 



Control 

 oil and 

 biscuit 



2cf 



142.5 

 71.9 

 57 



81.6 

 79.1 

 77.3 

 76.0 

 71.7 

 77.5 

 79.4 

 69.2 

 79.7 



Sudan 

 III 

 2cf 



109.5 

 33.9 

 57 

 82.1 

 78.6 

 77.2 

 76.7 

 72.7 

 76.7 

 79.8 

 76.0 

 78.1 



instance of atrophy may be due either to the abnormal nutri- 

 tional condition or possibly to a direct toxic action of Sudan III 

 on the thymus. 



I have given in table 4 (see also the last two columns in 

 tables 2 and 3) the weights of the organs determined in both 

 control and test rats at the end of the several experiments. 



The organs given in table 4 were used for the determination 

 of water and lipoid content, as well as for the demonstration of 

 the presence or absence of Sudan III in various organs, by both 

 microscopic and extraction methods. As we should expect, the 

 organs of the test rats are smaller than those of the controls. 

 It is interesting to note also that even the liver and pancreas, 

 although they have made a continuous growth during the ex- 

 perimental period, are yet far behind those of the control rats. 

 I wish to call special attention to the case of the control rats which 

 received the same amount of olive oil as the test rats (series IX 

 and X). In this case we notice that the control and test rats 

 show the same degree of difference in the organ weights as in 



