FEEDING SUDAN III TO YOUNG ALBINO RATS 



113 



In this table the means from all the twelve series of experi- 

 ments are given. We notice that in every instance the per- 

 centage of water in the Sudan III brain is less, and thus the dif- 

 ference in the water content between the control and the test rats 

 cannot be doubted. 



The full meaning of these alterations in the percentage of water 

 in the several organs I am unable to explain; nevertheless, it 

 seems evident that in the case of the liver the diminution of 

 water is mainly due to the accumulation of fat. From two 

 determinations I have obtained the following results for the 

 amount of fat in the liver: 



The histological examination shows clearly that the greater 

 part of the liver was infiltrated by fat. 



In the case of the brain I found a significant increase in the 

 amount of lipoid in the test rat, as will be seen from the following : 



This increase in lipoid may account for a slight diminution of 

 water content in the test rat, but this requires further study. 



A slight diminution of the water content in the case of the 

 kidneys and the spleen may also be due to a slight increase of 

 1 pin content as in the case of the brain, but I have no data to 

 prove this. The increased water content noted in the lungs 

 and pancreas I am also unable to explain at this moment. It 

 seems, however, highly probable that in the case of the pancreas, 

 hyperfunction in connection with the metabolism of fat may 

 account for this. 



THE JOURN.\L OF EXPERIMENTAL ZOOLOGY, VOL. 26, NO. I 



