FEEDING SUDAN III TO YOUNG ALBINO RATS 117 



3. Most organs maintain nearly their original weights; some 

 change in weight. The liver and pancreas show a steady in- 

 crease, while the thymus, testes, and ovaries show a striking 

 diminution. The rapidity of the thymus atrophy seems to be 

 proportional to the amount of Sudan III administered. 



4. The rats fed with Sudan III show a high degree of anaemia, 

 and the reduction of erythrocytes may be as high as 40 per 

 cent. 



5. The composition of the organs is more or less altered. We 

 find an increase of water content in the blood, lungs, and pan- 

 creas, while a reduction occurs in the liver, spleen, kidneys, 

 brain, and heart. 



6. The reduction in the water content of the brain belonging 

 to the Sudan Til fed rats is small, but it is highly uniform and 

 occurs in all of the- series without a single exception. It was 

 noted also that the brain belonging to the Sudan III fed rats 

 gives an alcohol-ether extract nearly 1.5 per cent greater than 

 in the control brain. 



7. The alcohol-ether extract of the dry total blood is signifi- 

 cantly greater (2.1 per cent) in the Sudan III fed rats than in 

 the controls. 



8. Extraction with ether shows a small trace of Sudan III 

 in the following organs: liver, pancreas, lungs, and kidneys, but 

 failed to show even a trace in the brain, spleen, or heart. 



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 CoRPER, H. J. 1912 Intra-vitam staining of tuberculous guinea-pigs with fat- 

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