134 CHARLES R. STOCKARD AND GEORGE N. PAPANICOLAOU 



A number of the treated animals have died and many others 

 have been killed at various times during the progress of the ex- 

 periment. Their organs and tissues have been carefully exam- 

 ined at autopsy and later studied microscopically. All tissues 

 have appeared practically normal and none of the various well- 

 recognized pathological conditions occurring in human alcoholism 

 have been discovered. Tissues from animals treated as long as 

 three years have been carefully studied, and the heart, stomach, 

 liver, lungs, kidney, and other organs present no noticeable con- 

 ditions that might not be found in normal individuals. Alco- 

 holized animals are usually fat, but no fatty accumulation has 

 been noted in the parenchyma of any organ. 



Several males and females have been semicastrated during 

 the experiment, and the ovaries and testes have been found to be 

 in a generally healthy condition. It has seemed, however, that 

 the ovaries of treated animals as well as all animals of the alco- 

 holic lines show an unusual tendency to become cystic as com- 

 pared with the ovaries of normal individuals. We have not, 

 however, made sufficient comparisons to give the foregoing 

 statement any greater weight than a mere supposition. 



The general condition of all animals under the fume treatment 

 is particularly good, and, as stated above, they continue to grow 

 if the treatment is begun on individuals before they have attained 

 full size, and all become fat and vigorous, taking plenty of food, 

 living long, and behaving in a typically normal way. 



The accompanying illustrations of five treated animals photo- 

 graphed along with control individuals show their perfectly 

 normal appearance. In figure 1 is seen two male guinea-pigs 

 and from the photograph as well as in life it would be impossible 

 for any one to detect signs of physical inferiority on the part of 

 one or the other. Yet the animal on the right. No. 80 cf, was 

 four days less than 5 years old when the photograph was made 

 and had inhaled alcohol over one hour per day, a sufficient dose 

 to give signs of intoxication, for six days per week, during four 

 years, two months and five days. During the last seven months 

 he had inhaled alcohol fumes two hours per day. He is per-- 

 fectly well and alert, as the photograph clearly shows. His 



