160 CHARLES R. STOCKARD AND GEORGE N. PAPANICOLAOU 



late generations, Fi to F4, on the basis of their productivity and 

 mortahty records. Such an analysis is of particular importance 

 to test in the first place whether the effects of the alcohol treat- 

 ment on the germ cells are permanent, altering their qualities in 

 inheritance, and in the second place whether an increasing 

 amount of normal germ plasm acquired with each generation 

 may tend to offset the original alcoholic effect by dilution. 



Table 2 contains the data from the non-inbred alcoholic lines 

 divided into different generations. The first vertical column 

 gives the records for 233 control young as a standard of com- 

 parison. These are the same records shown in the normal column 

 of table 1^ except that in the present table we have included in 

 the first horizontal line under each group the average birth weight 

 of the litters produced. This is termed the average litter weight 

 and is recorded in grams. For th^ normal stock this average 

 productivity is 197.12 grams; that is, the average weight of all 

 the litters at birth was this amount. The average litter weight 

 is in a way associated with the average litter size, since a litter 

 containing several young though each individual may not be so 

 large, will probably weight more than a litter of fewer or of one 

 young. Thus a group having a higher average litter than an- 

 other group will also probably have a higher average litter weight, 

 though this is not necessarily the case, as will be seen on com- 

 paring the several columns of the table. 



The second column contains the alcoholic line animals. This 

 again is the same 594 alcoholic animals shown in the second 

 column of table 1 and is given here for comparison with the four 

 following groups, each of which is a certain portion of this total 

 column. The average productivity for the alcoholic animals is 

 170 grams, or 37 grams less than the control, and when cor- 

 rected on the basis of the average litter size, it is 5.6 grams less 

 than it should be according to the normal standard. 



The third column gives the records of 186 animals with one or 

 both parents treated with alcohol, the Fi generation. Thirty- 

 three of these animals also had a slight alcoholic history in their 

 ancestry, and thus the entire group are not pure Fi alcoholics. 

 The proportion of large and small litters in this column is about 



