436 



W. C. ALLEE AND E. R. STEIN, JR. 



TABLE 4 



9/1/16. 1:20 p.m. Put two lots of eight May-fly nymphs each in aquarium water 

 in dark box. Light: 60-watt Mazda, 50 cm. distant. The nymphs had been in the 

 laboratory two days. Temperature aquarium 21 .5; of room 22. 



f< * Positive one Has been positive throughout put in biometer 2 : 20 with a 

 negative one that has been negative all the time. 



Washed with CO2 free air 5 minutes. 



Positive nymph in left chamber, negative in right. 



Bubble 2:25. 



First ppt. Rt. 2:28. 



Much more left 2:38. 

 Took out biometer, 2: 40. Both active; put back in dark box in separate dishes. 

 3: 00 Both reacting as before testing in biometer. 

 3:20 Do. 

 3:45 Do. 

 4: 10 Do. 



4: 15 Replaced in biometer as before. 

 4: 25 Bubble. 

 4: 27 First left, positive. 

 4: 40 More on left as before. 



4: 45 Out of apparatus. Reintroduced at negative end of separate dishes. 

 5:00 Nymph that had been positive throughout, still positive; other nega- 

 tive as before. 



6:00 Both negative. 



7:40 NjTnph negative throughout now positive, other negative. Light on 

 all night. 



8: 00 a.m. Both negative. 



