478 



ALVALYN E. WOODWARD 



lysin. To the filtrate Na2S04 is added in small quantities until 

 all the excess of barium is precipitated. The liquid remaining 

 can be saturated with (NH4)2S04 to precipitate the sperm 

 agglutinin. 



The work outhned on page 470 suggests that the egg secre- 

 tion consists of at least two substances. They have now been 

 separated, and hence we may consider the sperm agglutinin or 



TABLE 6 

 Lipolysin not specific as parthenogenelic agent 



1. Arbacia 



2. Arbacia 



3. Arbacia 



4. Arbacia 

 ■5. Arbacia 



6. Arbacia 

 utes 



7. Arbacia 



8. Arbacia 



9. Arbacia 



10. Arbacia 



11. Arbacia 



12. Arbacia 

 utes 



13. Arbacia 



14. Arbacia 



eggs, 

 eggs 

 eggs 

 eggs 

 eggs 

 eggs 



control 



+ sperm. . . 

 + Astei'ias 

 + Asterias 

 + Asterias 

 + Asterias 



lipolysin 1 hour 



lipolysin 1 hour + hyper 20 minutes . 



lipolysin Ik hours 



lipolysin 1| hours + hyper 20 min- 



eggs 

 eggs 

 eggs 

 eggs 

 eggs 

 eggs 



+ Asterias lipolysin 2 hours 



+ Asterias lipolj'-sin 2 hours + hyper 20 minutes . 



+ Arbacia lipolysin 1 hour , . . . 



+ Arbacia lipolysin 1 hour + hyper 20 minutes. 



+ Arbacia lipolysin Ik hours 



+ Arbacia lipolysin Ik hours + hj^pcr 20 min- 



eggs + Arbacia lipolysin 2 hours 



eggs + Arbacia lipoh^sin 2 hours + hyper 20 minutes 



Note. — The solution of lipolysin was made by dissolving 2 cc. of the powder, 

 shaken down in the bottom of a graduated centrifuge tube, in 15 cc. sea-water. 

 Nos. 3 to 8, inclusive, were made with freshly precipitated Asterias powder (one 

 week old); Nos. 9 to 14, with Arbacia precipitate ten months old. 



^ Estimated. 



spermophile group, as distinct from the lipolysin, or ovophile 

 group. The reason for Lillie's belief that fertilizin is an ambo- 

 ceptor is now clear. The effect of x-radiation on fertilizin and 

 the relation between efficiency and concentration of the secretion 

 suggest that the sperm agglutinin may be an enzyme. There is 

 slight evidence from these tests concerning the nature of the 

 parthenognetic agent. When we* actually apply to the pre- 

 cipitates tests for the individual classes of enzymes, however, it 



