EGG SECRETIONS OF ARBACIA AND ASTERIAS 



489 



been previously treated for one hour with 0.3 per cent solution of 

 ether in sea-water and then washed thoroughly. Moreover, 

 from four out of eleven females tested, the treated eggs, whether 

 mature or not, became surrounded by dense halos of sperm (see 



TABLE 14 

 The effect of lipoid solvents on resistant Asterias eggs 



Untreated controls 



Ether, 0.3 per cent 



60 minutes 



90 minutes 



Chloroform, saturated 



35 minutes 



48 minutes 



Chloroform, one-fourth saturated 



45 minutes 



60 minutes 



75 minutes 



Chloretone, 0.1 per cent 



35 minutes 



55 ^minutes 



90 minutes 



Chlotal hydrate, 0.1 per cent 



50 minutes 



2 hours 



3 hours 



Butyric acid, 0.5 cc. xf + 50 cc. sea- 

 water 



12 minutes 



20 minutes 



40 minutes 



Bi 



50 

 50 



50 

 50 



40 

 40 

 20 



25 

 25 

 25 



55 



50 



Few 



50 

 50 

 50 



D 



56 



E 



37 



17 



20 



15 



019 



Estimated. 



photographs). The others were unaffected. When, late in the 

 season, Asterias eggs were again resistant, this experiment was 

 repeated. Out of ten batches of eggs treated, every one attracted 

 sperm decidedly better than the control. 



