EPITHELIAL MOVEMENT 559 



d. Asbestos Jiber as support. Asbestos fibers were ignited and 

 treated with pure cone. HCl; the fine cloudy suspension was col- 

 lected and washed in distilled water to remove completely all 

 traces of HCl, and then sterilized before use. 



, In the serum culture of the cornea the fibers were mixed densely 

 so that, they appeared like a nest, in which the tissue lay. As 

 the fibers were very fine, they did not hinder microscopical exam- 

 ination, if not too densely placed. 



aiai2fiiai«j<-i3at'. 





Fig. 5 Experiment 233; 4. Same preparation as shown in fig. 4. Portion of 

 vertical section, di'awn from one of the serial sections, ep, epithelial surface of 

 cornea; c, connective tissue; ch, cartilaginous plate, placed on the endothelial 

 surface of cornea; t, cut end of cornea. Note the epithelial movement (E) on 

 the sclerotic cartilage (ch). Forty hours' growth. X 98. 



Thirty cultures were made in this group, with positive results in 

 twenty-two. Figure 8 shows an example of this series. 



e. Movement on pith, etc. Further studies were made with pith. 

 Thin pieces were used, which were kept in distilled water which 

 was changed every three days for a period of over three months. 



Experiments showed that the epithelium was able to cling to 

 the cell walls of the thin piece of pith. Figure 9 shows clearly 



