560 



SHINICHI MATSUMOTO 



the growing out of epithelial cells on the support. Out of twenty- 

 seven experiments nineteen gave positive results. 



On the shell membrane of hen's egg, used as support, after 

 thoroughly washing and boiling, a similar condition was observed. 



/ 



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gv/ 





■r»i 



/ 



Fig. 6 Experiment 238, 11. Epithelial movement in serum on spider web- 

 In the neighborhood of the explanted tissue (c) a good many isolated cells, round 

 in shape, were noticed, which are omitted in this figure; they showed no active 

 movement, t, edge of the piece; above, some cells move out in sheets on the 

 cover-glass. Compare Jour. Exp. Zool., 17, 521, figs. 4 to 7, 12. X 98. 



Fig. 7 Experiment 49, 7. Epithelial movement on glass wool (gw) ; culti- 

 vated four days in serum, t, edge of tissue. X 98. 



III. RESUME 



This paper deals with the movement of corneal epithelium of 

 the adult frog in vitro. Frog cornea is very suitable for this 

 purpose, as it is so transparent and thin that it permits of direct 

 observation of the cell movements. 



The corneal epithelium cultivated in plasma shows various 

 types of movement, according to the nature of the substratum 



