30 CHIKANOSUKE OGAWA 



Upon microscopical examination, the normal vesicles of the 

 two species give different impressions as to their characteristics 

 of form, at least in the specimens examined two weeks after 

 operation; but the transplanted vesicles seem to develop just the 

 same as those of the host and not to retain the exact character- 

 istics of those of the original animal. The same is true of the 

 cellular constitutents. For instance, there is an evident differ- 

 ence in the size of the cells in the two species, those of Ambly- 

 stoma being larger than the cells of Rana. 



Uhlenhuth ('13), in transplanting the eye from the larva of 

 Salamandra maculosa to another larva in a younger stage of 

 development, found that the transplant is not only delayed in 

 its metamorphosis, but that the metamorphosis is exactly syn- 

 chronous with that of the normal eye of the host, even to the 

 smallest detail. It seems to me that in my heteroplastic trans- 

 plantation the influence of the host upon the transplanted vesicle 

 was more than synchrony. Since my experiment was planned 

 for the study of inversion, it is not adequate for the solution of 

 this question. Therefore I shall make but a brief reference to 

 it and shall attempt to test its accuracy by further experiments and 

 more detailed observations. 



Transplanting vesicles from Rana to Ambly stoma 



Seven specimens were operated upon. In two development 

 was abnormal ; in two no vesicles were found upon later examina- 

 tion; in the remaining three cases complete rotation was estab- 

 lished. The results of this experiment, therefore, like those of 

 the previous one, fully justify the conclusion that environmental 

 influences can act upon ear vesicles of a different species. The 

 whole appearance and cellular constituents of the transplanted 

 vesicles appear to be the same as those of the host. 



Abnormal development seems to occur more frequently in 

 this than in the former experiment. It cannot be asserted 

 positively whether this is due to mere accident or to difficulty in 

 transplantation from Rana to Amblystoma; that is, from a 

 higher to a lower species. 



