484 E. J. LUND 



Experiments 1 and 2 were discontinued before it was possible 

 to decide what regenerative processes, if any, would finally 

 have occurred in ends toward the cathode and those turned 

 toward the anode, which showed no regeneration. For this 

 reason experiment 2 was repeated. • 



Experiment 3. The current density was slightly greater 

 than in experiment 2. This is also clearly evident when the 

 results in branch I, experiment 2, is compared with branch I 

 in this experiment. The material was obtained from vigorous 

 growing colonies on the laboratory float. Duration of the 

 experiment was sixty-six hours, thus allowing time enough for 

 regeneration of stolons, if these would develop in the pieces. 



Observations were made at intervals throughout the experi- 

 ment. At thirty-one hours the difference in growth between 

 ends toward the anode on basal and apical internodes was 

 very clearly shown in branches I, II, and III. A very much 

 greater growth occurred toward the anode than toward the 

 cathode in the same branches. Only traces of growth, if any, 

 appeared in branch IV. The greater orienting effect of the 

 stronger current in this experiment was plainly evident when 

 compared to that in experiment 2 at the same interval. 



At the end of sixty-six hours (plate 3, A, B), branch IV in 

 relative current density of 1 shows practically complete inhibition 

 at both ends of the internodes. Only two immature polyps had 

 developed on apical ends toward the anode, which without the 

 current would have reached full development in less than forty 

 hours. Two undeveloped stolons appeared at ends toward the 

 cathode. 



Branch III regenerated stolons on all internodes which showed 

 any appreciable growth on the cathode end. 



Hydranths formed only on the anode ends of the pieces, and 

 apical internodes in general developed hydranths earlier than 

 basal internodes of the same branch. This therefore shows that, 

 irrespective of whether the basal or apical end of the inter- 

 node is turned toward the anode, stolons can develop on the 

 end toward the cathode. 



