REGENERATION AFTER EXARTICULATION 119 
regeneration of posterior limbs in the tadpole of the midwife- 
toad (Alytes obstetricans). The new skeleton was “normal 
or nearly so’ in five cases. Byrnes (’04) using frog-tadpoles 
showed that the anterior limbs would regenerate while still 
under the operculum but the new limb is invariably smaller than 
normal and there is a tendency to reduction in the skeletal 
elements. Morgan (’08) (and Goldfarb) attempted to induce 
regeneration in the fore-leg of the adult frog by artificial means. 
Pieces of the leg, muscle and other tissues from the tail of the 
tadpole were grafted into the stump but with only small success. 
In some cases, however, incomplete regeneration of the leg with 
rudimentary toes was obtained, or a broad flat ‘foot’ with scant 
toes. Histological details were not given. Glaeser (10) more 
recently tested the power of regeneration in the hind limbs of 
adult frogs but found none except in two cases where a ring of 
peripheral (periosteal) cartilage developed around the stump of 
the femur. No artificial means were used to induce regeneration 
in this case. 
Part II 
To test further the power of regeneration in Diemyctylus a 
series of more complicated operations were made, involving 
losses not usually met with under natural conditions. These 
are to some extent a repetition of those of Wendelstadt (’01) 
and Reed (03) but with certain modifications. 
Experiment 1. Hxtirpation of the fibula and a portion of the 
femoral epiphysis but without injury to the tarsus. Number of 
animals, ten:—Of these two escaped and one lost the foot. The 
remaining seven were killed at intervals of from forty-eight 
days to one year. There was no indication of regeneration of 
a new fibula, but the lost portion of the femoral epiphysis was 
restored. 
_Expervment 2. Extirpation of the fibula without injury to 
either femoral epiphysis or tarsus. Number of animals, ten:— 
These were killed at intervals of from thirty-three days to one 
year. For the most part no indication of regeneration was 
observed but in two specimens there was a narrow mass of ¢al- 
