8 PORTER. [VoL. XIV. 
The fate of the spores after their escape is still unknown. 
They are so minute that it would be almost impossible to iden- 
tify them in the tissues of the host. I have found, however, 
imbedded in the wall of the intestine of Clymenella, on the 
body-cavity side, numerous large amoeboid cells, evidently 
foreign organisms (Pl. II, Figs. 34-36); these, I think, may 
possibly be the early amoeba-like stage of this gregarine devel- 
oped directly from spores, but I have seen no intermediate 
stages. 
After trying many methods of killing and many kinds of 
stains, I found that the best results were invariably obtained 
by killing in a concentrated aqueous solution of corrosive 
sublimate, and staining in Kleinenberg’s haematoxylin. The 
aqueous stains were, as a rule, unsatisfactory, although I suc- 
ceeded very well with Mayer’s aqueous haemalum. 
Notwithstanding the greatest care in hardening the specimens 
and in passing them gradually into chloroform or into oil of 
thyme, I could not prevent the sporocysts from shrinking more 
or less. Pl. II, Fig. 33, represents a portion of a section 
through a nearly mature gregarine cyst; it shows sections 
through sporocysts in various directions and the great amount 
of shrinkage that has taken place. 
The best way that I have found for preparing slides of sporo- 
cysts consists in staining the gregarines zz zofo and breaking 
open the cysts with needles after they have been transferred to 
balsam ready for mounting. 
About one-fourth of all the worms I have examined were 
infested. I have collected Clymenella as late as the last of 
November and as early as the middle of April, and have never 
failed to find infested worms. 
My material was collected at Woods Holl, and at the mouth 
of the Saugus River, near Lynn, Massachusetts. 
II. A Gregarine from Rhyncobolus. 
While collecting Clymenella I frequently dug up fine speci- 
mens of Rhyncobolus Americanus, Verrill. Curiosity led me 
to search the intestines of a few of these, where, to my sur- 
