No. I.] TWO NEW GREGARINIDA. 7 
many experiments with a dozen or more different stains did I 
discover that there was nothing left to stain, that the cysts 
containing the spores had escaped from the capsules and had 
been washed away. 
I have not been able to determine how either of the envelopes 
is formed, but they are both undoubtedly secreted by the proto- 
plasm of the sporogonium, the capsule first, the cyst afterwards. 
The protoplasm in the cyst gradually collects into cord- or 
band-like structures apparently in connection with the elon- 
gated nuclei, of which they appear as though tail-like appen- 
dages (Pl. II, Figs. 22, 23). The bands at first are only feebly 
stainable; later they stain more deeply. Large globules of a 
colorless substance appear in the cyst at the same time; finally, 
the protoplasm becomes entirely consumed in the formation of 
the tails. 
At the time of the first appearance of the tail-like proto- 
plasmic portions of the spores, the nuclei are elongated, cylin- 
drical rods, usually constricted slightly in several (3 to 5) places, 
so as to present a rather evenly monilate form. With the com- 
pletion of the protoplasmic portion of the spore, the chromatic 
mass becomes shorter and somewhat thicker, and stains more 
deeply than formerly. It finally assumes an appearance as 
though made up of only two oval bodies of equal size united 
end to end. Each portion is about twice as long as thick, and 
the union of the two is often so intricate that there is only a 
trace or even no evidence of a constriction, and the nucleus 
then is a single oval body some three or four times as long as 
thick. 
The tail portion of the immature spores is apt to contain 
vacuoles, and to shrink somewhat in the process of hardening 
(PE Il, Fig. 27). The spores represented in Pl. IJ, Fig: 28; are 
abnormal, or else have been subjected to too great pressure. 
In many of the sporocysts I have found the spores arranged 
in a regular manner, with their head ends, that is, their nucleated 
ends, in or near the equatorial plane, four of their tails being 
turned in one direction and four in the opposite. In fully as 
many cases, however, the spores had apparently no definite 
arrangement in the cyst (PI. II, Fig. 32). 
