No. 1.] TWO NEW GREGARINIDA. 3 
compact and finely granular condition. Thus at f7’gl" (Pl. I, 
Fig. 6) the protoplasm is still extensively vacuolated, while at 
prpl' this, more properly speaking, frothy condition has given — 
place to fine granules, some of which are shown highly magni- 
fied in Pl. I, Fig. 8. The compact condition of the protoplasm 
at the close of this stage is shown by the numerous cracks run- 
ning through hardened specimens (Pl. I, Fig. 7). The cracks 
are due, of course, to the method of hardening. 
I believe that the disintegration of the nucleus continues 
until the chromatic matter no longer exists as such; at any rate, 
until it becomes divided into particles so fine as to be invisible 
even under the highest powers of the microscope. One of the 
reasons for believing that a complete dissolution of the nucleus 
takes place is that with its disappearance the chemical char- 
acter of the protoplasm seems to change. I believe that the 
protoplasm then develops slightly acid qualities, for I have 
found that it acts on the haematoxylin stain, turning it gradually 
to a reddish tint, while the tissue enveloping the cyst retains 
its original blue color. This is very noticeable in my old slides, 
where all the cysts in this stage of development stand out as 
red spots from their blue surroundings (PI. I, Fig. 7). 
Very frequently the first sporogonia are formed before the 
network state of the protoplasm has been completely replaced 
by the finely granular condition. The protoplasm shrinks 
away from the wall of the cyst and then sends out homogene- 
ous, translucent, amoeboid processes from the whole or part of 
its periphery (Pl. I, Figs. 9, 10). The granules of the central 
mass are carried out through these processes and, collecting 
together in clusters, help to form the sporogonia (PI. I, Fig. 11). 
Meanwhile numerous chromatic particles appear, which, by 
arranging themselves into rings (Pl. I, Figs. 11, 14, and Pl. I, 
Fig. 18), form the nuclei of the sporogonia. 
As more and more sporogonia are formed, the central mass 
of protoplasm becomes smaller and smaller, large vacuoles 
appear, and sporogonia are likewise seen in these (Pl. I, 
Fig. 12). A fine filamentous network can be distinguished in 
the cyst (ji/, ri/, Pl. I, Fig. 12); it is perhaps a useless rem- 
nant of the original protoplasmic mass. 
