Volume XIV. September, 1598. Number 3. 
JOURNAL 
OF 
MOR Hi@ tO 
BUDDING IN PEROPHORA.! 
GEORGE LEFEVRE. 
ALTHOUGH the phenomenon of budding in the group of 
Tunicata has received the attention of numerous observers for 
a long time, during the last twelve or fifteen years investigation 
has been largely confined to the process in the pelagic forms, 
Salpa, Doliolum, and Pyrosoma. In the ascidians, however, 
what we have known of the budding, until recently, has been 
based almost entirely on the observations of earlier workers. 
Among the latter Kowalewsky (12 and 13), who studied the 
bud development in Perophora, Didemnum, and Amaroucium, 
Della Valle (4), in Didemnum, Distaplia, and Botryllus, and 
finally Seeliger (29) and Van Beneden and Julin (33), in Clave- 
lina, are especially prominent. 
Within the last three or four years, however, our knowledge 
has been greatly enriched by the researches of Pizon (22) and 
Hjort (8) on Botryllus, of Salensky (27) on Distaplia, and by 
those of Caullery (1). Although the observations of these 
investigators agree in many important points, still, in others, 
notably the origin of the nervous system, great difference of 
opinion exists. 
1 This paper was accepted as a dissertation for the degree of Doctor of Philosophy | 
by the Board of University Studies of the Johns Hopkins University, May, 1896. 
