On the Histogenesis of Gastric Glands. 479 
K6pschis a good fixative for Bensley’s three color stain (see below). 
Controls were often made with Zenker and 5 per cent freshly 
distilled formalin, but these only served to emphasize the superior- 
ity of Bensley’s fluid, especially for the neutral gentian and copper 
chrome stains. The eosinophile granules of the parietals, the 
zymogenic granules and the contents of mucigenous cells are 
especially well preserved by this fluid. 
Sections were cut 4 “ thick, and fixed to slide by the water 
method, or by Mayer’s albumin. Demercurization was completed 
by immersion of slides in iodine-alcohol. 
Hematoxylin and eosin stains were used in each stage for com- 
parison, but the main reliance was placed upon the four stains 
named below, serial sections of each stage being stained with 
each of these: 
1. Bensley’s three color stain as described by Klein (06) p. 
323. The granules of parietal cells are stained a vivid cherry-red 
and the intercellular ductules are brought out well. This has 
proved the best all around stain for stomach material. 
2. Neutral gentian (Bensley ’00) in 20 per cent alcoholic 
solution was invaluable in determining the appearance of zymo- 
genic granules. It is also, as far as my experience goes, the best 
cement line stain, and is thus of great value in the study of the 
parietal ductules, and of cell boundaries. The material should 
never be brought into water, as the zymogen granules then disap- 
pear. I stained two to four days. 
3. Bensley’s Copper chrome hematoxylin as described by Har- 
vey (07, p. 209). The granules of the parietals are stained a 
steel blue or deep blue black. The chromatin stains black, but 
is more readily destained than are the parietal granules. 
4. Mayer’s Muchematin, as modified by Bensley (’03, p. 11) for 
the detection of mucigen. It is best to avoid the water and heat 
method in fastening sections to slide, as the granular form of 
the mucigen is thereby altered. 
