504 Edwin G. Kirk. 
been able, by Golgiimpregnation, although I have tried many times, 
to demonstrate, even in the latest embryonic stages, any contin- 
uity between these vacuoles and the ductules. 
After 7 cm. mitoses are very frequent in the fundus tubules, 
occurring at all depths, but especially at the very bottom and at 
the juncture of the first (upper) and second quarter, 7. e., in the 
territory corresponding in the adult, to the lower part of the fove- 
ola. Most are in theadelomorphs, nevertheless parietalsare often 
caught in mitosis. At 8-9 em., every tubule of the fundus 
region has acquired some parietal cells. At 9 cm., and later, pari- 
etals are found with constricted nucleus. These I interpreted 
as examples of amitosis. Possibly they presagea cessation of cyto- 
plasmic division. In later stages (20-29 em.) multinucleate parie- 
tals appear. After 10-11 em. the actual and relative increase of 
parietals in the cervical region, and relative decrease in the bottom 
of the tubule is marked. The latter is due to the proliferative 
activity of the undifferentiated gland cells, by which the tube 
is constantly lengthening downward. Mitoses also occur on the 
processes but not so frequently. 
At 9-10 cm., no mucus is present in any fundic cell. At 12- 
13 em., this is yet true for the large part of the fundus, but, near 
the pylorus, along the greater curve, very shallow, distal goblets 
of mucus appear in the cells of the surface and upper quarter of 
the tubule. At 15 cm. in this part of the fundus, the surface 
cells have acquired quite deep goblets, while in the upper third 
of the tubule are shallower goblets, and a few mucous chief cells. 
In the stages 15—20em. this mucous differentiation of surface and 
foveolar cells spreads rather gradually to the other parts of the 
fundus.?! 
Thus, even at 17 em. we find a condition not far advanced, 
in this respect, over that of 13 em.,—the surface and foveolar 
cells of some areas presenting but narrow rims of mucus, and the 
neck chief cells staining but faintly, or not at all, in muchematin. 
‘This occurs with striking slowness as compared with its rapidity in the pylo- 
rus. Moreover, in the latter, the gland cells at the very bottom were the first to 
manufacture mucin, the pyloric surface cells of some embryos showing no mucus 
as late as 12-18 cm, but the gland mucus chief cells staining heavily. 
