PHYSIOLOGICAL PROPERTIES OF GONADS 



135 



The transplantations were made on young animals twenty- 

 six to fifty-seven days after birth. The procedure was usually 

 as follows : 



A male and a female were etherized at the same time, the 

 coelomic cavity of both exposed, and one sex gland of each 

 removed, the second gland remaining undisturbed; the extirpated 

 gland furnished the material for the transplant to the rat of 



TABLE 1 



SERIAL NUMBER 



AGE OF ANIMAL 

 AT GRAFTING 



AGE OF GRAFT WHEN 

 ANIM.VL WAS KILLED 



NUMBER OF GRAFTS 

 PERSISTING 



Males ivith ovarian graft 



1 Not sectioned. 



opposite sex. An ovary from the female was cut into two pieces, 

 these being placed subcutaneously, intramuscularly, or intra- 

 peritoneally in the male; and in like manner the testis material, 

 cut into small pieces, provided the graft material to be placed 

 in the female. SHght injury to the tissue at the site of trans- 

 plantation produced a local hyperemia and with the small- 

 sized pieces of the graft more chance was provided for the estab- 

 lishment of vascular connections. The animals were kept in 

 an incubator for from twenty-four to thirty-six hours after 



