PHYSIOLOGICAL PROPERTIES OF GONADS 157 



indication is not definite proof that such a process is going on; 

 no germ cells aside from those included within a follicle have 

 come to my attention. 



There is no trace of a corpus luteum in any of the sections of 

 the graft nor is there any evidence that ovulation has ever taken 

 place. 



Animal 49 A. Male rat, born Nov. 6, '17. Dec. 1, the right testis 

 was removed (used to graft into a female) and two pieces of ovary 

 were grafted subcutaneously into abdominal fascia and muscle. Aug. 

 22, '18, animal killed; both ovarian grafts had persisted, the left testis 

 was normal, sperm sacs were normal. Both ovarian grafts sectioned. 



Microscopic observations. Graft no. 1. After its residence 

 in the male host, possessing one testicle, for a period of 232 days 

 after transplantation, the ovarian graft shows characteristic 

 ovarian tissue. Both medulla and cortex are perfectly distinct, 

 as well as sections of the oviduct carried over into the male 

 at the time of operation. There are many Graafian follicles of 

 various stages of growth and one is able to follow the follicular 

 history from the primordial follicle through its various stages, 

 including follicles whose cavities are just appearing, follicles 

 with well-formed cavities and perfectly distinct discus proligerus 

 with its enclosed ovum, follicles undergoing atresia in which the 

 cavity is large, or those having progressed so far that only the 

 indication of the old follicular cavity remains. These masses 

 are surrounded by the connective-tissue stroma and the granulosa 

 cells have reverted to a lipoid-containing stage characteristic of 

 the interstitial cells; a very great number of this character are 

 present. 



This graft is the smaller of the two and consists of but about 

 ninety sections. 



Graft no. 2. The ovarian tissue proper of this graft (after a 

 residence in the male of 232 days) is composed of about 230 

 sections (cut lO^t thick). The tissue, which is accompanied by 

 a small piece of the oviduct, is characteristic in most of its 

 structures. Medulla and cortex are distinct, the stroma is 

 characteristic, and all stages in the life-history of graafian follicles 

 are abundantly represented. By actual count, forty-six normal 



