162 CAEL E. MOOEE 



granulosa is beginning to show an abnormal condition. In 

 this, as well as in all other graft sections of the entire series, the 

 interstitial cell masses are relatively abundant. 



B. The testis 



Animal 40-4 A2B2 III. Female, born Nov. 14, 1917. Dec. 18, 

 right ovary removed, two small pieces of testis from a brother rat were 

 grafted subcutaneously. May 18, '18, animal killed; left ovary nor- 

 mal, oviduct, and uterus normal, both grafts persisted, one sectioned. 



Serial sections of the graft show the testis material imbedded 

 between layers of muscle tissue. During the healing of the 

 wound after the operation, connective tissue grew into and around 

 the graft so that at first sight it presents the appearance of 

 a loosely arranged connective tissue within which are located the 

 conspicuous seminiferous tubules. The tubules are quite niuner- 

 ous, the number of sections varying as the majority of the 

 tubules are cut in cross or longitudinal sections. 



Figure 13 is a reproduction of a portion of one section and 

 shows the characteristics of the degenerate tubules; this tubular 

 mass is continuous in 420 sections of the graft taken from the 

 female animal. Of the tubules themselves, besides the con- 

 nective-tissue framework, there are a few relatively large cells 

 inside the tubular walls scattered indefinitely amid a reticular, 

 non-cellular material; these are the cells that are commonly 

 spoken of as the remaining Sertoli cells. 



The intertubular spaces are occupied by connective tissue, 

 the two kinds of foreign cells mentioned previously, and some 

 interstitial cells. The chief feature to consider, however, is that 

 there is no evidence of a hypertrophy of the interstitial gland 

 of the. testis in any of these grafts, but one must consider that 

 the tubular mass is not enclosed by its tunica albuginea (only a 

 small piece of the testicle having been used in the transplanta- 

 tion), hence the interstitial cells, not being confined, would 

 probably have been more easily scattered by the ingrowth of 

 connective tissue. It may be that this difference may account 

 for the difference in results of these and Steinach's graft material, 

 in reference to the quantity of interstitial cells, and that in 



