376 CARL R. MOORE 



show the condition of the mammary glands eight months after 

 the original ovarian transplantation. Figure 1 B shows for com- 

 parison the same structures of a normal male animal ; in the latter 

 the teats, one on each side, are scarcely visible, while on the oper- 

 ated male they are very prominent though reduced somewhat in 

 size from their condition of two months earlier. 



As indicated above, eight pieces of ovary were grafted into the 

 animal in two separate operations.* Before death two subcu- 

 taneous grafts could be palpated and after death these two sub- 

 cutaneous grafts as well as two intraperitoneal grafts were re- 

 covered from the animal; vascularization was well established 

 in all. 



B. Females with testis grafts 



In transplantation of pieces of guinea-pig testis a smaller num- 

 ber of grafts grew or persisted for an appreciable length of time, 

 but the effects of the grafts were somewhat more apparent than 

 in ovarian transplantation, especially when considering the ac- 

 companying psychical effect. Following simple ovariotomy, the 

 female guinea-pig is, without exception in my experience, sexually 

 indifferent; such an animal's reactions give no indication of male- 

 ness or femaleness. Accompanying growth of testicular grafts 

 in a completely spayed female, however, the animals, as they 

 become adults, react typically as males both toward each other 

 and toward other animals. 



Though testicular transplantation was made in fifteen or 

 eighteen female guinea-pigs following complete ovariotomy, in 

 some cases of which the animal received three or four successive 

 grafts at intervals of from five days to a week, there were but 

 two animals in which the grafts persisted long enough to study 

 their effect. In each of these animals (67 Bl and 68 Bl) a single 

 operation was made fifteen and eighteen days after birth, respect- 

 ively, when the ovaries were removed and two pieces of testis 

 grafted subcutaneously. Animals 67 Bl was killed March 3, 1919, 

 nine months after the transplantation was made, and both grafts, 



* The young ovary, used as material for the grafts, was cut into two pieces 

 with scissors, so that in all four ovaries were implanted in this animal. 



