136 GEORGE W. CORNER 



The collection of this material would have been impossible 

 without the special and unusual cooperation which has been ex- 

 tended to this laboratory by the Western Meat Company of San 

 Francisco. I refer to their donation of permanent laboratory 

 quarters in their West Berkeley plant (Oakland Meat and Pack- 

 ing Company) to the Anatomical Laboratory of this institution. 

 I owe especial thanks to Mr. J. 0. Snyder, general superintendent 

 of the Western Meat Company, and to Mr. Ralston B. Brown, 

 superintendent of the Oakland Meat and Packing Company, 

 and to many other members of the staffs and employes of both 

 these establishments; to Dr. H. H. Hicks, U. S. Supervising In- 

 spector, Dr. G. R. Ward, and other members of the U. S. Inspec- 

 tion Service at the South San Francisco plant, and to Dr. Thomas 

 Presst, of the California State Inspection Service. To Mr. R. 

 B. Brown in particular I owe the opportunity of observing living 

 animals and of obtaining their pelvic organs, often at the cost, 

 I fear, of some inconvenience to the routine of his plant. The 

 permanent laboratory space provided by him at the packing- 

 house has been invaluable during the prosecution of this work. 

 I am further indebted to Professors Evans and Sabin for the con- 

 tribution of ovaries with the corresponding early embryos; to 

 Prof. J. I. Thompson, of the Department of Agriculture of the 

 University of California, for observing and marking five animals, 

 and to Messrs. A. E. Amsbaugh and Felix H. Hurni for assistance 

 in the collection and preparation. 



In general the younger specimens were fixed in Bouin's fluid, 

 the older in 10 per cent formol, these fluids being selected to 

 secure the advantage of fixation in slow aqueous coagulants, as 

 will be explained in the next section; small pieces of many 

 ovaries were placed in osmium tetroxide for study of the lipoids. 

 Blocks were imbedded in paraffin and celloidin. The chief 

 stains used with the specimens herein described comprised 

 haematoxylin and eosin, Heidenhain's iron haematoxylin, Mal- 

 lory's triple connective-tissue stain, Van Gieson's mixture, and 

 several lipoid-soluble dyes (Nile-blue sulphate, Sudan III, Schar- 

 lach R), besides many special procedures applied to fresh and 

 fixed tissues. 



